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The AlphaLISA® SureFire® Ultra™ total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization
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The Alpha SureFire® Ultra™ Multiplex phospho-p70 S6K (Thr389) + Total p70 S6K assay kit is used to measure both the phosphorylation (on Thr389) and total levels of endogenous p70 S6K in cellular lysates. The assay
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This positve control lysate is prepared from MCF7 cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 500 μg/mL Insulin for 30 minutes and lysed in 20 mL of
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